Aromatase-positive Sphingomonas|392214 |BNCC

Essential Information Related Products

Sphingomonas haloaromaticamans

Culture medium	BNCC352246
Description	R2A Agar
Composition	Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K₂HPO₄:0.3g, MgSO₄:0.024g, Sodium Pyruvate(C₃H₃NaO₃):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃)
Growth conditions	30°C; 24-48h; aerobic
Subculture procedure	① Prepare 1-2 fresh plates as described above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it, and subsequently crush it with forceps; ③ Pipette 0.5mL of liquid medium into a freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under specified conditions; strain is ready upon growth.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter 1-2mm, circular, with neat edges; opaque, grayish-white upper surface; central elevation; smooth, glossy surface; moist texture; easily lifted; G- (red); bacillus; purity: pure
Sharing mode	Public welfare sharing