BeNa Culture Collection
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| Culture medium | BNCC363892 |
| Description | Modified PYG Broth |
| Composition | Pancreatic Digest of Casein:5.0g, Peptone:5.0g, Yeast Extract:10.0g, Beef Extract:5.0g, Glucose:5.0g, K2HPO4:2.0g, Tween 80:1.0ml, CaCl2.2H2O:0.01g, MgSO4.7H2O:0.02g, K2HPO4:0.04g, KH2PO4:0.04g, NaHCO3:0.4g, NaCl:0.08g, Resazurin:1.0mg, Hemin:5mg, Vitamin K1:1mg, C3H7NO2S.HCl:0.5g, pH:7.2±0.2(25℃) |
| Growth conditions | anaerobic at 37 ° C, 48-72 hours |
| Subculture procedure | ① Dissolve the Frozen vial in a 37 ° C water bath and rapidly shake for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into the liquid test tube culture medium (the culture medium is deoxygenated 24 hours in advance); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ° C |
| Safety level | 2 |
| morphology | G(+), Cocci |
| Sharing mode | Public welfare sharing |
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