Morganella morganii subsp. morganii|139556 |BNCC

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Morganella morganii subsp. morganii

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30°C; 18-24 hours; aerobic;
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in biosafety cabinet, cauterize top with alcohol lamp, then quickly add sterile water to shatter; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into freeze-dried vial, dissolve completely, then return to liquid tube and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, irregularly margined, opaque, pale in color, centrally convex, smooth surface, glossy appearance, moist texture, G− (red), bacillus
Sharing mode	Public welfare sharing