BeNa Culture Collection
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| Culture medium | BNCC353703 |
| Description | 2216E agar |
| Composition | Peptone:5.0g, Yeast Extract:1.0g, Ferric Citrate(FeC6H5O7):0.1g, KBr:0.08g, NaCl:19.45g, MgCl2:5.98g, Na2SO4:3.24g, CaCl2:1.8g, KCl:0.55g, Na2CO3:0.16g, Na2NO3:0.0016g, Na2HPO4:0.008g, SrCl2:0.034g, H3BO3:0.022g, Na2SiO3:0.004g, NaF:0.0024g, Agar:15.0g, pH:7.4-7.8(25℃) |
| Growth conditions | 25°C; 48-72h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder thoroughly, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5–1 mm; circular, with neat margins; opaque, grayish-white upper surface; convex center; smooth, shiny surface; moist texture; easily liftable; G− (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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