BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC353703 |
| Description | 2216E agar |
| Composition | Peptone:5.0g, Yeast Extract:1.0g, Ferric Citrate(FeC6H5O7):0.1g, KBr:0.08g, NaCl:19.45g, MgCl2:5.98g, Na2SO4:3.24g, CaCl2:1.8g, KCl:0.55g, Na2CO3:0.16g, Na2NO3:0.0016g, Na2HPO4:0.008g, SrCl2:0.034g, H3BO3:0.022g, Na2SiO3:0.004g, NaF:0.0024g, Agar:15.0g, pH:7.4-7.8(25℃) |
| Growth conditions | 25°C; 48-72h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder thoroughly, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5–1 mm; circular, with neat margins; opaque, grayish-white upper surface; convex center; smooth, shiny surface; moist texture; easily liftable; G− (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
KMS11(KMS11)
BNCC340591
TJ905(TJ905)
BNCC339987
OCI-AML-5(OCI-AML-5)
BNCC341619
NCI-H2286(NCI-H2286)
BNCC342561
Py230(Py230)
BNCC378460
SN-12C(SN-12C)
BNCC396361