BeNa Culture Collection
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| Culture medium | BNCC352243 |
| Description | LB nutrient agar |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0±0.2(25℃) |
| Growth conditions | 30°C; 18-24h; aerobic; |
| Subculture procedure | ① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat, opaque edges; light color; raised center; smooth, shiny surface; moist texture; easily liftable; G+ (blue-purple); bacillus |
| Sharing mode | Public welfare sharing |
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