Pasteur Yeast|140792 |BNCC

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Saccharomyces pasteurianus

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	28°C; 24-48h; aerobic;
Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder thoroughly, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5-1 mm; circular with irregular margins; opaque; milky white on top; centrally convex; smooth surface; glossy appearance; moist texture; viscous consistency; G+ (blue-violet); cocci; Purity: Pure
Sharing mode	Public welfare sharing