Yellow lipophilic bacillus|360213 |BNCC

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Corynebacterium lipophiloflavum

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the lyophilized vial, dissolve the bacterial powder completely, then dispense approximately 200μL onto each of two plates; spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, with neat, opaque edges; grayish-white upper surface; central convexity; glossy, smooth surface; moist texture; G+ (blue-violet); bacillus
Sharing mode	Public welfare sharing