White-covered black egg nest|150431 |BNCC

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Cyathus pallidus

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28°C; 7-10 days; aerobic;
Subculture procedure	① Prepare 1-2 plates as above; ② Disinfect the surface of test tube slants before opening in a biosafety cabinet; ③ Cut blocks (see appendix) into 0.5×0.5 cm² squares using a sterile inoculating fork and spatula; ④ Place the small square block flat onto the center of the Agar plate, on the agar surface; ⑤ Incubate the Agar plate upright under the specified conditions; the strain is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Small filamentous fungus; distinct colonies on comprehensive PDA medium; mycelium on the Agar plate surface is grayish-white, spreading toward the Agar plate edge; underside of the medium is yellow.
Sharing mode	Public welfare sharing