Aspergillus fumigatus|151891 |BNCC

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Aspergillus fumigatus

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi, with obvious colonies on Chatelier agar medium, gray white, dense and vigorous velvet like hyphae, and later produce light yellow
Sharing mode	Public welfare sharing

Aspergillus penicilloides

Storage conditions : 2~8 ℃

No. : 151891

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, Cha's agar medium, 5-7 days. Cha's agar medium: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of sodium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water, pH 6.2 & plusmn;0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Place the plate under the above culture conditions, and the strain can be used when it grows out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows

Item	test results
viability:	good viability, in 5days strain layer become obvious
colony morphology:	Small filamentous fungi, with obvious colonies on Cha's agar medium, gray-white, dense and vigorous velvety hyphae, later producing light yellow spores, spreading and growing to the edge of the plate, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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