BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 24-48h; anaerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Use forceps to break it into pieces; ③ Pipette 0.5mL sterile water into the lyophilized vial. After fully dissolving the bacterial powder, dispense approximately 200μL onto each of two plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Size: 2-4mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: Grayish-white Elevation: Flat Surface: Dull Texture: Moist |
| Sharing mode | Public welfare sharing |
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