Clostridium sporogenes|360712 |BNCC

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Clostridium sporogenes

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 24-48h; anaerobic
Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Use forceps to break it into pieces; ③ Pipette 0.5mL sterile water into the lyophilized vial. After fully dissolving the bacterial powder, dispense approximately 200μL onto each of two plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	2
morphology	Size: 2-4mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: Grayish-white Elevation: Flat Surface: Dull Texture: Moist
Sharing mode	Public welfare sharing