BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 30°C; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the lyophilized vial, dissolve the bacterial powder completely, then dispense approximately 200μL onto each of two plates, spreading evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Round, with neat edges; opaque, grayish-white on top, dark-colored; centrally convex, smooth surface, glossy appearance, moist texture, easily lifted; non-pigmented, G− (red), bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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