PAO1 Pseudomonas aeruginosa Luc tagged plasmid type|360258 |BNCC

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PAO1Pseudomonas aeruginosa Luc marker plasmid type

Culture medium	BNCC360873
Description	LB Medium with 50μg/mL Streptomycin
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions	37 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	Round, with neat edges, opaque, white on the front and back, smooth and bright on the surface, moist texture, easy to pick up, non pigmented, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing