BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 30°C; 5-7 days; aerobic; |
| Subculture procedure | ① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Small filamentous fungus. Colony formation is distinct on comprehensive PDA medium. The Agar plate layer initially exhibits white mycelium, with edges turning pale yellow later. Produces white spores in the later stage. The medium appears pale yellow on the reverse side. |
| Sharing mode | Public welfare sharing |
Escherichia coli WM3064(Escherichia coli WM3064)
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Streptococcus sp. Grouping strain H60R(Streptococcus sp. Grouping strain H60R)
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Lactobacillus taiwanensis M9-1(Lactobacillus taiwanensis M9-1)
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Burkholderia pyrrocinia 2327(Burkholderia pyrrocinia 2327)
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Burkholderia cenocepacia YC40(Burkholderia cenocepacia YC40)
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Azotobactervinelandii(Azotobactervinelandii)
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