Enterobacter faecalis|359789 |BNCC

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Odoribacter splanchnicus

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 3-5 days; anaerobic;
Subculture procedure	① Prepare 1-2 fresh plates as above (pre-placed in anaerobic environment for 24h to remove oxygen); ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium or sterile water (pre-deoxygenated in anaerobic conditions for 24 hours) into the freeze-dried vial, dissolve completely, and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension into the Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate under the specified conditions. The strain is ready for use once colonies appear.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 0.5–1 mm; circular; edges irregular; opaque; grayish-white upper surface; raised center; smooth surface; glossy appearance; moist texture; easily lifted; Gram-negative (red); bacillus
Sharing mode	Public welfare sharing