BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 5-7 days; Micro aerobic (3% -5% O2, 10% CO2); |
| Subculture procedure | ① Prepare one of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of Brucella broth and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate and apply evenly; Cover the agar surface with sufficient (1-4ml) Brucella broth or brain extract broth to maintain biphasic culture throughout the entire cultivation period (the culture will not grow on a dry surface). ④ Transfer to the above cultivation conditions for cultivation. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, semi transparent, protruding in the middle, smooth surface, G - (red), rod-shaped, purity: pure |
| Sharing mode | Public welfare sharing |
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