BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37°C; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush contents with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. Thoroughly dissolve powder, then dispense 200 μL/well onto plates, spreading evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat margins; opaque, grayish-white upper surface; central convexity; glossy, smooth texture; moist consistency; easily liftable; Gram-negative (red); bacillus |
| Sharing mode | Public welfare sharing |
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