Escherichia coli|359420 |BNCC

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Megasphaera elsdenii

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 48-72h; anaerobic
Subculture procedure	① Thaw Frozen vials in water bath (fungi 30°C, bacteria 37°C), dissolve by rapid shaking; ② Disinfect outer surface of vial with alcohol swab, then transfer to biosafety cabinet for handling; ③ Unscrew cap, aspirate entire thawed contents, and inoculate 1-2 agar plates (approx. 200μL per Agar plate; for anaerobic bacteria, pre-deoxygenate medium in anaerobic conditions for 24h); ④ After uniform spreading, incubate under the specified conditions.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, with neat margins; opaque, white upper surface; central elevation; smooth, glossy surface; moist texture; easily liftable; G− (red); cocci; Purity: Pure
Sharing mode	Public welfare sharing