Neisseria meningitidis A|359480 |BNCC

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Neisseria meningitidis A

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 18-24h; 5% CO₂
Subculture procedure	① Prepare two plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Crush ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. Thoroughly dissolve bacterial powder, then inoculate onto 2 plates (200 μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 0.5–1 mm; circular, with neat, opaque edges; grayish-white upper surface; central elevation; smooth texture; easily liftable; Gram-negative (red); cocci
Sharing mode	Public welfare sharing