BeNa Culture Collection
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| Culture medium | BNCC362653 |
| Description | GYM Streptomyces Agar |
| Composition | Glucose:4.0g, Yeast Extract:4.0g, Malt Extract:10.0g, CaCO3:2.0g, Agar:15.0g, pH:7.2(25℃) |
| Growth conditions | 28°C; 48-72h; aerobic |
| Subculture procedure | ① Prepare two plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. Thoroughly dissolve bacterial powder, then inoculate two plates (200 μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; irregular, opaque edges; white upper surface with central elevation; rough texture; dry consistency; difficult to pick/cut; Gram-positive (blue-violet); bacilli; purity: pure |
| Sharing mode | Public welfare sharing |
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