Weigert's Broth|358903 |BNCC

Essential Information Related Products

Scardovia wiggsiae

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 3-4 days; anaerobic;
Subculture procedure	① Prepare 1-2 fresh plates as described above (pre-deoxygenated in anaerobic environment for 24 hours); ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water (pre-deoxygenated in anaerobic conditions for 24h) into the lyophilized tube, dissolve completely and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate under the specified conditions. The culture is ready for use once the strain grows.
Storage conditions	-80°C
Safety level	1
morphology	Colony diameter: 0.5-1 mm, circular, irregular edges, opaque, white upper surface, raised center, G+ (blue-violet), bacillus, purity: pure
Sharing mode	Public welfare sharing