Bacteroides subsp. guanii|356083 |BNCC

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Parabacteroides goldsteinii

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 3-4 days; anaerobic
Subculture procedure	① Prepare 1-2 fresh plates as above (pre-placed in anaerobic environment for 24h oxygen removal); ② Open in biosafety cabinet, cauterize top with alcohol lamp, then quickly add sterile water to rupture; break into pieces with forceps; ③ Pipette 0.5mL liquid medium or sterile water (pre-placed in anaerobic environment for 24h oxygen removal) into lyophilized vial, dissolve completely and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension into the agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate under the specified conditions. The culture is ready for use once the bacterial strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular shape with neat edges; opaque; grayish-white upper surface; raised center; smooth texture; moist consistency; easily lifted; Gram-negative (red); bacillus
Sharing mode	Public welfare sharing