Lactobacillus paracasei|356106 |BNCC

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Lactobacillus iners Falsen et al. 1999

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 48-72h; anaerobic;
Subculture procedure	① Prepare two plates as above (equilibrate in anaerobic environment for 24h); ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Crush contents with forceps; ③ Pipette 0.5 mL sterile water (pre-equilibrated anaerobically for 24h) into freeze-dried vial. Thoroughly dissolve powder, then inoculate two plates (200 μL/Agar plate) with uniform smears; ④ Incubate plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, irregularly margined, opaque; upper surface grayish-white, flat morphology, rough texture; Gram-positive (blue-violet), bacilli; purity: pure
Sharing mode	Public welfare sharing