BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 48-72h; anaerobic; |
| Subculture procedure | ① Prepare two plates as above (equilibrate in anaerobic environment for 24h); ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Crush contents with forceps; ③ Pipette 0.5 mL sterile water (pre-equilibrated anaerobically for 24h) into freeze-dried vial. Thoroughly dissolve powder, then inoculate two plates (200 μL/Agar plate) with uniform smears; ④ Incubate plates under the specified conditions. The culture is ready for use once bacterial growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular, irregularly margined, opaque; upper surface grayish-white, flat morphology, rough texture; Gram-positive (blue-violet), bacilli; purity: pure |
| Sharing mode | Public welfare sharing |
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