Nitrosomonas macrophylla|357823 |BNCC

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Azomonas macrocytogenes

Culture medium	BNCC360815
Description	Nitrogen fixing medium
Composition	KH₂PO₄:0.2g, K₂HPO₄:0.8g, MgSO₄.7H₂O:0.2g, CaSO₄.2H₂O:0.1g, FeCl₃:Trace, Na₂MoO₄.2H₂O:Trace, Yeast Extract:0.5g, Mannitol:20.0g, Agar:15.0g, pH:7.2(25℃)
Growth conditions	28 ° C; 24-48 hours; aerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, moist texture, G - (red), oval shape, purity: pure
Sharing mode	Public welfare sharing