Anomalous Rhizopus|356130 |BNCC

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Absidia anomala

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28°C; 5-7 days; aerobic;
Subculture procedure	① Prepare 1-2 plates as above; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then promptly add sterile water to rupture it. Use forceps to break it into pieces; ③ Pipette 0.5mL sterile water into the lyophilized tube. After fully dissolving the powder, dispense approximately 200μL onto each of two plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Small filamentous fungi produce distinct colonies on PDA medium. Mycelium appears white, cottony, dense, and vigorous, spreading toward Agar plate edges to cover the entire surface. The underside of the medium turns pale yellow.
Sharing mode	Public welfare sharing