Kubica mycobacteria|357610 |BNCC

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Kubica mycobacteria-BNCC
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  • Mycobacterium kubicae-BNCC

Mycobacterium kubicae

  • Price： $ 223
  • number：BNCC357610
  • MPs：
    The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, yellow on the front, raised in the middle, smooth on the surface, moist texture, G+(blue purple), Bacillus, purity: pure
  • Packing：Freeze dried pellet; Bacterial suspension; Agar plate
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Mycobacterium kubicae
Culture medium BNCC360851
Description Middlebrook 7H10 Agar
Composition (NH4)2SO4:0.5g, KH2PO4:1.5g, Na2HPO4:1.5g, Na3C6H5O7:0.4g, MgSO4:0.025g, CaCl2:0.5mg, ZnSO4:0.001g, CuSO4:0.001g, Sodium L-Glutamate(C5H8NNaO4):0.5g, C6H8FeNO7:0.04g, Pyridoxine Hydrochloride(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50℃), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25℃)
Growth conditions 37 ℃; 5-7 days; aerobic
Subculture procedure ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, transfer it back to the liquid test tube and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Storage conditions 2-8 ℃
Safety level 2
morphology The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, yellow on the front, raised in the middle, smooth on the surface, moist texture, G+(blue purple), Bacillus, purity: pure
Sharing mode Public welfare sharing
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