Escherichia coli|356167 |BNCC

Essential Information Related Products

Muribaculum intestinale

Culture medium	BNCC382470
Description	Tryptic Soy Blood Agar with Hemin and Vitamin K₁ (TSA-HK)
Composition	Tryptone:15.0g, Phytone:5.0g, NaCl:5.0g, Sterile defibrinated sheep blood:5%, Agar:15.0g, Hemin:5mg, Menadione(Vitamin K3):0.5mg, pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 3-7 days; anaerobic
Subculture procedure	① Dissolve the frozen tube in a water bath (37 ℃ for bacteria) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	The colony has a diameter of 0.5-1mm, irregular shape, irregular edges, opaque, gray white front, raised middle, smooth surface, bright surface, moist texture, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing