BeNa Culture Collection
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| Culture medium | BNCC352238 |
| Description | MRS agar medium |
| Composition | Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃) |
| Growth conditions | 30°C; 24-48h; aerobic; |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a biosafety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to rupture it. Use forceps to break it into pieces; ③ Pipette 0.5 mL sterile water into the freeze-dried vial. After thoroughly dissolving the bacterial powder, dispense approximately 200 μL onto each of two plates and spread evenly; ④ Incubate plates under the specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Size: 1-2 mm Shape: Circular Edge: Neat Transparency: Opaque Color: Pale yellow Elevation: Central dome Surface: Bright and smooth Texture: Moist and easily lifted |
| Sharing mode | Public welfare sharing |
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