BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 24-48h; aerobic |
| Subculture procedure | ① Prepare 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it open; use forceps to crush it; ③ Pipette 0.5mL sterile water into the freeze-dried vial and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto the Agar plate and spread evenly; repeat twice to obtain two plates; ⑤ Incubate all plates under specified conditions until colonies develop. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular, irregularly margined, opaque; upper surface white, centrally convex, glossy, dry texture; G+ (blue-violet), cocci; purity: pure |
| Sharing mode | Public welfare sharing |
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