Escherichia coli (E. coli)|335833 |BNCC

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Escherichiacoli(Migula)CastellanietChalmers

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C; 18-24h; Aerobic;
Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder thoroughly, then dispense 200 μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once colonies appear.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, with neat edges; opaque, grayish-white upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; non-pigmented; Gram-negative (red); bacillus; purity: pure
Sharing mode	Public welfare sharing