Purple Bacillus|241534 |BNCC

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Chromobacterium violaceum Bergonzini

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it. Use forceps to crush it; ③ Pipette 0.5mL liquid medium into the freeze-dried vial. After complete dissolution, transfer back to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspensioninto each Agar plate, spread evenly, and repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Storage conditions	2-8°C
Safety level	2
morphology	Size: 1-2mm
Sharing mode	Public welfare sharing