Streptomyces genus|335904 |BNCC

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Streptomyces sp.

Culture medium	BNCC352247
Description	Gaoshi Synthesis 1 Agar
Composition	Soluble Starch:20.0g, NaCl:0.5g, FeSO₄:0.01g, KNO₃:1.0g, K₂HPO₄:0.5g, MgSO₄:0.5g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	28°C; 24-48h; aerobic
Subculture procedure	① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then transfer back to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm, circular, with neat edges; opaque, grayish-white on top, flat in shape, rough surface, glossy appearance, firm texture, difficult to pick/cut; Gram-positive (blue-violet), filamentous; Purity: Pure
Sharing mode	Public welfare sharing