BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352251 |
| Description | YM agar medium |
| Composition | Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃) |
| Growth conditions | 28°C; 24-48h; aerobic; |
| Subculture procedure | ① Prepare 1 test tube containing 5-10 mL liquid medium and 2 plates; ② Open in biosafety cabinet, flame top with alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into freeze-dried vial, dissolve completely, then return to liquid test tube and mix thoroughly; ④ Pipette 0.2mL of bacterial suspension onto each Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Size: 2-4mm Shape: Circular Edge: Neat Transparency: Opaque Color: Creamy white Elevation: Central dome Surface: Smooth and glossy Texture: Viscous and moist |
| Sharing mode | Public welfare sharing |
UACC-812(UACC-812)
BNCC338200
H22(H22)
BNCC338327
HCC-78(HCC-78)
BNCC338064
NCI-H1373(NCI-H1373)
BNCC341744
HepAD38(HepAD38)
BNCC341745
NCI-H1693(NCI-H1693)
BNCC342281