Rhizopus niger|336302 |BNCC

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Rhizopus nigricans Ehrenberg

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28°C; 5-7 days; aerobic;
Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in biosafety cabinet, flame top with alcohol lamp, then quickly add sterile water to shatter; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into freeze-dried vial, dissolve completely, then return to liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension onto each Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Filamentous fungi produce distinct colonies on comprehensive PDA medium, exhibiting rapid growth. Hyphae are grayish-white, densely packed, tall, and spreading to cover the entire Agar plate. Later stages produce black spores.
Sharing mode	Public welfare sharing