BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 30°C; 18-24h; aerobic; |
| Subculture procedure | ① Prepare 1 test tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Size: 2-4mm Shape: Wavy Edge: Irregular Transparency: Opaque Color: Pale yellow Elevation: Flat Surface: Roughly wrinkled Texture: Dry and difficult to pick up |
| Sharing mode | Public welfare sharing |
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Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
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Plasmid DNA of Necrotizing Hepatopancreatitis (NHP)(Plasmid DNA of Necrotizing Hepatopancreatitis (NHP))
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