Lactobacillus plantarum|336981 |BNCC

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Sporolactobacillus inulinus

Culture medium	BNCC352238
Description	MRS agar medium
Composition	Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO₄:0.2g, CH₃COONa:5.0g, C₆H₈O₇.(NH₄)₂:2.0g, K₂HPO₄:2.0g, MnSO₄:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions	Culture temperature 37°C; Incubation time 2-3 days; anaerobic gas environment;
Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates (pre-deoxygenated in anaerobic environment for 24 hours); ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 0.5-1mm Shape: Circular Edge: Irregular Transparency: Opaque Color: Milky white Elevation: Central dome Surface: Bright and smooth Texture: Moist and viscous
Sharing mode	Public welfare sharing