BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28°C; 3-5 days; aerobic; |
| Subculture procedure | ① Prepare 1 test tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Small filamentous fungus. Colony formation is distinct on comprehensive PDA medium with rapid growth. Mycelium appears as white, cottony growth spreading to cover the entire Agar plate. Produces yellow spores in later stages, turning the medium surface yellowish-brown. |
| Sharing mode | Public welfare sharing |
Escherichia coli WM3064(Escherichia coli WM3064)
BNCC395800
Streptococcus sp. Grouping strain H60R(Streptococcus sp. Grouping strain H60R)
BNCC395802
Lactobacillus taiwanensis M9-1(Lactobacillus taiwanensis M9-1)
BNCC395799
Burkholderia pyrrocinia 2327(Burkholderia pyrrocinia 2327)
BNCC395783
Burkholderia cenocepacia YC40(Burkholderia cenocepacia YC40)
BNCC395737
Azotobactervinelandii(Azotobactervinelandii)
BNCC395776