Bacillus subtilis|347725 |BNCC

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Bacillus pumilus

Culture medium	BNCC353703
Description	2216E agar
Composition	Peptone:5.0g, Yeast Extract:1.0g, Ferric Citrate(FeC₆H₅O₇):0.1g, KBr:0.08g, NaCl:19.45g, MgCl₂:5.98g, Na₂SO₄:3.24g, CaCl₂:1.8g, KCl:0.55g, Na₂CO₃:0.16g, Na₂NO₃:0.0016g, Na₂HPO₄:0.008g, SrCl₂:0.034g, H₃BO₃:0.022g, Na₂SiO₃:0.004g, NaF:0.0024g, Agar:15.0g, pH:7.4-7.8(25℃)
Growth conditions	30°C; aerobic; 24h;
Subculture procedure	① Prepare 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve completely, and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto the Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, with neat edges; opaque, light-colored; raised center; smooth, shiny surface; moist texture; easily liftable; G– (red); bacillus; purity: pure
Sharing mode	Public welfare sharing