BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 18-24h; 5% CO₂ |
| Subculture procedure | ① Prepare two plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately break with sterile water; shatter contents with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube, dissolve powder completely, then inoculate 2 plates (200 μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1–2 mm; circular, with neat, opaque edges; grayish-white upper surface; smooth texture; moist consistency; non-pigmented; Gram-positive (blue-violet); cocci; purity: pure |
| Sharing mode | Public welfare sharing |
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