Aspergillus flavus|356062 |BNCC

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Aspergillus flavus

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	37°C; 5-7 days; aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL liquid medium and two plates; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then transfer back to the liquid test tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Storage conditions	2-8℃
Safety level	1
morphology	Small filamentous fungus. Colony formation is distinct on YGC medium. Initial mycelium is white, dense, and vigorous, later producing yellow spores that cover the Agar plate.
Sharing mode	Public welfare sharing