BeNa Culture Collection
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| Culture medium | BNCC352232 |
| Description | Tryptic Soy Broth |
| Composition | Tryptone:17.0g, Soy Peptone:3.0g, NaCl:5.0g, K2HPO4:2.5g, Glucose:2.5g, pH:7.3±0.2(25℃) |
| Growth conditions | 30 ℃; 24-48 hours; aerobic; |
| Subculture procedure | ① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Colonies are 1-2 mm in diameter, circular with entire margins, and opaque. The colony surface is smooth, moist, glistening, and convex in the center. They appear dark yellow in color, are easily emulsifiable, and do not produce diffusible pigments. Microscopic examination reveals Gram-negative (staining red) bacilli. |
| Sharing mode | Public welfare sharing |
Escherichia coli WM3064(Escherichia coli WM3064)
BNCC395800
Streptococcus sp. Grouping strain H60R(Streptococcus sp. Grouping strain H60R)
BNCC395802
Lactobacillus taiwanensis M9-1(Lactobacillus taiwanensis M9-1)
BNCC395799
Burkholderia pyrrocinia 2327(Burkholderia pyrrocinia 2327)
BNCC395783
Burkholderia cenocepacia YC40(Burkholderia cenocepacia YC40)
BNCC395737
Azotobactervinelandii(Azotobactervinelandii)
BNCC395776