BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; gas environment 5% CO2+95% air |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each plate contains approximately 200 μL, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After spreading evenly, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The colony has a diameter of 1-2 mm, is circular with neat edges, opaque, grayish-white on the front, convex in the center, smooth in texture, bright in appearance, moist in quality, easily lifted, Gram-negative (red), coccus-shaped, and pure in purity |
| Sharing mode | Public welfare sharing |
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