BeNa Culture Collection
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| Culture medium | BNCC359963 |
| Description | Trypticase Soy Blood Agar |
| Composition | Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Sterile Defibrinated Sheep Blood:5%, Agar:15.0g, pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48h；5%CO2； |
| Subculture procedure | ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into the freeze-drying tube, mix thoroughly and thoroughly; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
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