BeNa Culture Collection
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| Culture medium | BNCC360852 |
| Description | EG Medium |
| Composition | Beef Extract Powder:2.4g, Proteose Peptone:10.0g, Yeast Extract Powder:5.0g, Na2HPO4:4.0g, Glucose:1.5g, Soluble Starch:0.5g, L-Cystine:0.2g, Agar:15.0g, L-Cysteine Hydrochloride(C3H7NO2S.HCl):0.5g, Sterile Horse Blood:50mL, pH:7.6-7.8(25℃) |
| Growth conditions | 37 ℃; 48-72h; anaerobic; |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each plate contains approximately 200 μL, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After spreading evenly, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | No inventory available |
| Sharing mode | Public welfare sharing |
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