Bacillus amyloliquefaciens|188070 |BNCC

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Bacillus amyloliquefaciens

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30°C; 18-24h; aerobic
Subculture procedure	① Prepare one test tube containing 5–10 mL liquid medium and two plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 1-2mm Shape: Circular Margins: Irregular Transparency: Opaque Color: Grayish-white Elevation: Flat Surface: Rough and dull Texture: Dry
Sharing mode	Public welfare sharing