Aspergillus oryzae|341636 |BNCC

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Aureobasidium pullulans

Culture medium	BNCC352233
Description	Potato glucose agar
Composition	Potato Infusion Powder:6.0g, Glucose:20.0g, Agar:20.0g, pH:5.6±0.2(25℃)
Growth conditions	28 ℃; 7-10 days; aerobic;
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃.
Safety level	1
morphology	Fungi similar to yeast, with obvious colonies on PDA medium, yellow in color. After 10 days, black substance is produced at the edge of the colony, and there are light yellow black dots on the back of the medium
Sharing mode	Public welfare sharing