Aspergillus oryzae|341636 |BNCC

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Aureobasidium pullulans

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 7-10 days; aerobic;
Storage conditions	2-8 ℃.
Safety level	1
morphology	Fungi similar to yeast, with obvious colonies on PDA medium, yellow in color. After 10 days, black substance is produced at the edge of the colony, and there are light yellow black dots on the back of the medium
Sharing mode	Public welfare sharing

Description

1. Name: Aureobasidium pullulans

2. BNCC No.: 341636

3. Biosafety level: 4

2.Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, PDA agar: potato boil 1.0L, glucose 20.0g, agar 15.0g, natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

2. Atmosphere: aerobic

3, Temperature: 25-28 ℃, about 10 days

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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