Bacillus subtilis subsp. subtilis|231928 |BNCC

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Bacillus subtilis subsp.subtilis

Culture medium	BNCC352243
Description	LB nutrient agar
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0±0.2(25℃)
Growth conditions	37°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspensioninto each Agar plate, spread evenly, and repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 2-4mm Shape: Irregular Edges: Pseudohyphal Transparency: Opaque Color: Grayish-white Elevation: Flat Surface: Rough and dull Texture: Dry and easily lifted
Sharing mode	Public welfare sharing