Bacillus subtilis|336389 |BNCC

BeNa Culture Collection

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Bacillus subtilis-BNCC
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  • Bacillus subtilis(Ehrenberg)Cohn-BNCC

Bacillus subtilis(Ehrenberg)Cohn

  • Price： $ 186
  • number：BNCC336389
  • MPs：
    Size: 2-3 mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: Grayish-white Elevation: Flat Surface: Dull and rough Texture: Dry and difficult to pick up
  • Packing：Agar Slant; Bacterial suspension; Agar plate
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Essential Information Related Products
Bacillus subtilis(Ehrenberg)Cohn
Culture medium BNCC352239
Description Nutrient Agar
Composition Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions 30°C; 18-24h; aerobic;
Subculture procedure ① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, flame the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2 mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Storage conditions 2-8°C
Safety level 1
morphology Size: 2-3 mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: Grayish-white Elevation: Flat Surface: Dull and rough Texture: Dry and difficult to pick up
Sharing mode Public welfare sharing
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