Penicillium chrysogenum|192154 |BNCC

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Penicillium chrysogenum-BNCC
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  • Penicillium aurantiogriseum-BNCC

Penicillium aurantiogriseum

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  • number：192154
  • Form：
    Small filamentous fungus. Colony is distinct on comprehensive PDA medium. Mycelium is deep gray-green, velvety, later producing dense, vigorous dark gray spores. Grows vigorously toward the Agar plate edge. Underside of medium turns golden yellow.
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Penicillium aurantiogriseum
Subculture procedure ① Prepare 1 test tube containing 5-10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then transfer back to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Growth conditions 28°C; 7-10 days; aerobic
Storage conditions 2-8°C
Safety level 1
morphology Small filamentous fungus. Colony is distinct on comprehensive PDA medium. Mycelium is deep gray-green, velvety, later producing dense, vigorous dark gray spores. Grows vigorously toward the Agar plate edge. Underside of medium turns golden yellow.
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Description 

Name:  Penicillium aurantiogrise

BNCC No.: 192154 

Biosafety level: 4

Storage conditions :

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions ：

1. Comprehensive PDA: potato boiled juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 25 ℃

Notes : 
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.
 

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