BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352238 |
| Description | MRS agar medium |
| Composition | Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃) |
| Growth conditions | 37°C; 48-72h; anaerobic |
| Subculture procedure | ① Prepare two plates as described above; ② Disinfect the ampoule surface, open it in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Break it open with forceps; ③ Aspirate approximately 0.5 mL sterile water (previously equilibrated in anaerobic conditions for 24 hours) into the freeze-dried vial. Thoroughly dissolve the bacterial powder, then inoculate 2 plates with 200 μL each, spreading evenly; ④ Incubate plates under the specified conditions. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat margins; opaque; white upper surface with central convexity; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
KMS11(KMS11)
BNCC340591
TJ905(TJ905)
BNCC339987
OCI-AML-5(OCI-AML-5)
BNCC341619
NCI-H2286(NCI-H2286)
BNCC342561
Py230(Py230)
BNCC378460
SN-12C(SN-12C)
BNCC396361
Canid herpesvirus 1 nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397975
Lactobacillus intestinalis DNA standard
BNCC397805
Acholeplasma laidlawii DNA standard
BNCC397769
Mycoplasma bovis DNA standard
BNCC397625
Acinetobacter lwoffii nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397508
Lachnoclostridium edouardi DNA lab quality control
BNCC397450
- English
- Japanese