Bacillus sphaerospermos|194789 |BNCC

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Sphingobacterium sp.

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	30°C; 18-24h; aerobic
Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried tube, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grayish-white Elevation: Central dome Surface: Bright and smooth Texture: Moist and easily lifted
Sharing mode	Public welfare sharing